A. Presence of active pseudopodia.
C. Presence of intracytoplasmic vacuole.
D. Presence of two nucleoli.
The most distinctive feature of pathogenic Entamoeba histolytica on fresh stool examination is Erythrophagocytosis.
The presence of red blood cells in the cytoplasm (erythrophagocytosis) is diagnostic of E. histolytica as it is the only intestinal amoeba to exhibit this characteristic.