Dry Heat Sterilization

Dry Heat Sterilization


Dry Heat Sterilization 

Mechanism of Action

  • Protein denaturation
  • oxidative damage and toxic effects of elevated levels of electrolytes
Types

Flaming

  • For sterilizing inoculating loop or wire tip of forceps and searing spatulas

Burning or incineration

  • For contaminated cloth
  • Hospital dressing is best disposed by incineration
  • animal carcasses
  • pathological materials
  • PVC and polythene
  • Human anatomical waste
  • Cytotoxic drug
  • incineration and disposed of by secured landfills. 
  • Polystyrene should autoclave
  • Three basic kinds of incineration technology are of interest for treatment of healh care wastes 
  1. Double-chamber pyrolytic incinerators : designed to burn infectious health care waste.
  2. Single-chamber furnaces with static grate
  3. Rotary kilns operating at high temperatures:genotoxic substances and heat-resistant chemicals. 

Hot air oven:

  • Most widely used method of sterilization by dry heat:
  • The holding period of 160oC for one hour is used to sterlize:
  • glassware
  • scalpels
  • all glass syringes
  • swabs
  • liquid paraffins
  • dusting powder
  • fats
  • grease
  • For cutting instrument
  • Temperature of 150oC for 2 hours is required
  • 170oC for holding period of 20 minutes.
  • A rapid method:
  • 190 °C (374 °F) for 6 minutes for unwrapped objects 
  • 12 minutes for wrapped objects

Drawback:

  • lt has no Penetrating power so not used for bulky articles such as mattresses

Sterilization control of dry heat:

  • Done by spore of nontoxigenic strain of Cl. tetani
Exam Question
 

Burning or incineration

  • For contaminated cloth
  • Hospital dressing is best disposed by incineration
  • animal carcasses
  • pathological materials
  • Human anatomical waste
  • Cytotoxic drug
  • incineration and disposed of by secured landfills. 
  • Double-chamber pyrolytic incinerators :designed to burn infectious health care waste.

Hot air oven:

  • The holding period of 160oC for one hour is used to sterlize
  • glassware,
  • scalpels
  • all glass syringes
  • swabs
  • liquid paraffins
  • dusting powder
  • fats
  • grease
  • For cutting instrument
  • Temperature of 150oC for 2 hours is required
  • 170oC for holding period of 20 minutes.
Don’t Forget to Solve all the previous Year Question asked on Dry Heat Sterilization

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