Denaturation Of Dna

Denaturation of DNA

Q. 1

Thermo-stability in DNA is contributed mostly by

 A

A=T

 B

G=C

 C

Molecular base

 D

Parallel arrangement

Q. 1

Thermo-stability in DNA is contributed mostly by

 A

A=T

 B

G=C

 C

Molecular base

 D

Parallel arrangement

Ans. B

Explanation:

Base Composition.
More GC pairs more the Tm.


Q. 2

DNA denaturation is measured by absorbance at

 A

220nm

 B

230nm 

 C

240 nm

 D

260nm

Q. 2

DNA denaturation is measured by absorbance at

 A

220nm

 B

230nm 

 C

240 nm

 D

260nm

Ans. D

Explanation:

Increase in the optical absorbance of UV light by Purine and Pyrimidine bases called hyperchromicity.( DNA bases absorbs UV light of 260nm).



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