Denaturation of DNA
Q. 1
Thermo-stability in DNA is contributed mostly by
| A |
A=T |
|
| B |
G=C |
|
| C |
Molecular base |
|
| D |
Parallel arrangement |
Q. 1
Thermo-stability in DNA is contributed mostly by
| A |
A=T |
|
| B |
G=C |
|
| C |
Molecular base |
|
| D |
Parallel arrangement |
Ans.
B
D
Explanation:
Base Composition.
More GC pairs more the Tm.
Q. 2
DNA denaturation is measured by absorbance at
| A |
220nm |
|
| B |
230nm |
|
| C |
240 nm |
|
| D |
260nm |
Q. 2
DNA denaturation is measured by absorbance at
| A |
220nm |
|
| B |
230nm |
|
| C |
240 nm |
|
| D |
260nm |
Ans.
Explanation:
Increase in the optical absorbance of UV light by Purine and Pyrimidine bases called hyperchromicity.( DNA bases absorbs UV light of 260nm).
