E. coli Diagnosis and Treatment

E. coli Diagnosis and Treatment

Q. 1

Which of the following acts as defence against E.coli?

 A

Lactoglobulin

 B

Lactalbumin

 C

Lactoferrin

 D

None of the above

Q. 1

Which of the following acts as defence against E.coli?

 A

Lactoglobulin

 B

Lactalbumin

 C

Lactoferrin

 D

None of the above

Ans. C

Explanation:

Lactoferrin binds to iron and makes it unavailable for E.coli and acts a defence mechanism against infections.
 
Ref: Nutrition and Child Development, K.E. Elizabeth, 4th edition pg: 17

 


Q. 2

Labile toxin of E.coli can be detected by the following methods of incubation except ‑

 A

Into infant rabbit bowel

 B

Into adult rabbit skin

 C

Infra gastrically into infant Mouse

 D

Into Tissue Culture of Chinese Hamster ovary cells

Q. 2

Labile toxin of E.coli can be detected by the following methods of incubation except ‑

 A

Into infant rabbit bowel

 B

Into adult rabbit skin

 C

Infra gastrically into infant Mouse

 D

Into Tissue Culture of Chinese Hamster ovary cells

Ans. C

Explanation:

Ans. is ‘c’ i.e., lntra gastrically into infant mouse

Methods for detection of ETEC enterotoxins

Assay

LT

ST

In vivo tests

±

+

+

‑

Ligated rabbit deal loop

Read at 6 hours

Read at 18 hours

Infant rabbit bowel

+

+

Infant mouse intragastric (4 hours)

+

Adult rabbit skin (vascular permeability)

+

‑

In vitro tests

 

 

Tissue culture tests

 

 

Rounding of Y1 mouse adrenal cells

 

 

Elongation of chinese hamster ovary (CHO) cells

+

‑

Serological tests

 

 

ELISA

+

(ST-ELISA with

monocolonial antibody)

Passive agglutination tests, passive immune

 

hemolysis, precipitin (Eiken’s) test

+

 

Genetic tests

 

+

DNA probes

+

 



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