Question
All are true about CRISPR cas 9 Except
A. gRNA ensures that the Cas9 enzyme cuts at the right point in the genome
B. NHEJ repair & HDR pathway
C. C9 enzyme is used in CRISPR gene editing
D. All of these
Show Answer
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Correct Answer » D
Explanation
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Answer-D. All of these
CRISPR-Cas9 was adapted from a naturally occurring genome editing system that can generate double standard Breaks (DSBs).
Cas9 is the enzyme that is used most often, other enzymes (for example Cpf1) can also be used→ these enzymes can can repair by homologus repair system or by non-homologous end joining in the absence of DNA template.
Two general repair pathways:
The efficient but error-prone non-homologous end joining (NHEJ) pathway
The less efficient but high-fidelity homology directed repair (HDR) pathway
