Question
Restriction endonuclease cuts at
A. AAGGAA
B. AAGAAG
C. AAGTTC
D. AAGCTT
Show Answer
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Correct Answer » D
Explanation
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Answer-D. AAGCTT
* AAGCTT is the only palindrome among the choices. Because the sequence of only one DNA strand is given, one must determine the base sequence of the complementary strand. To be a palindrome, both strands must have the
* The vast majority of restriction endonucleases recognize palindromes, and
same sequence when read in the 5’→3′ direction. Thus, the complement of 5′-AAGCTT-3′ is also 5′-AAGCTT-3′.
