Separation techniques of protein
| A | SDS-PAGE | |
| B |
Ultracentrifugation |
|
| C |
Affinity chromatography |
|
| D |
HPLC |
Proteins are separated on the basis of charge in ‑
| A |
SDS-PAGE |
|
| B |
Ultracentrifugation |
|
| C |
Affinity chromatography |
|
| D |
HPLC |
Ans. is ‘d’ i.e., HPLC
Molecular size of protein is determined by‑
| A |
Sedimentation |
|
| B |
SDS-PAGE |
|
| C |
Salting out |
|
| D |
Lyophilization |
Ans. ‘B’ SDS PAGE
Molecular size is determined by
- Gel filtration chromatography (molecular-sieve chromatography or size exclusion chromatography)
- Ultracentrifugation
- SDS-PAGE (Sodium dodecyl sulfate-polyacrylamide gel electrophoresis)
- Dialysis
| A | Western blot | |
| B |
Affinity electrophoresis |
|
| C |
Thin-layer chromatography |
|
| D |
None |
- The most conventional and widely used high-throughput methods for protein-protein interactions (PPI) are yeast two-hybrid screening and affinity purification coupled to mass spectroscopy.
Other methods used to study PPI are:-
- LI Affinity electrophoresis
- Co-immunoprecipitation
- Protein microarrays
- Analytical ultracentrifugation
- Light scattering
- Fluorescence spectroscopy
- SPINE (strep-protein interaction experiment)
- LUMIER (luminescence-based mammalian interactome mapping)
- Calorimetry
- QUICK (quantitative immunoprecipitation combined with knock-down)
- BiFC (Biomolecular fluorescence complementation)
- PLA (Proximity ligation assay)
- Resonance energy transfer system
- Mammalian protein-protein interaction trap
- Surface plasma resonance
- Protein-fragment complementation assay
- Tandem affinity purification
Which of the following method of protein separation is not dependent on molecular size ‑
| A |
Gel filtration chromatography |
|
| B |
Ultracentrifugation |
|
| C |
Ion-exchange chromatography |
|
| D |
SDS-PAGE |
In this method, the separation is based on electrostatic attraction between charged biological molecules to oppositely charged groups on the ion exchange resins. These resins are cross-linked polymers containing ionic groups as part of their structure. The polymer must be sufficiently cross-linked to have negligible solubility, but porous enough for the ions to diffuse freely through it.
