Question
Single base-pair substitution is related to which of the following?
A. RFLP
B. PCR
C. FISH
D. Southern blot
Show Answer
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Correct Answer » A
Explanation
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The term RFLP (Restriction Fragment Length Polymorphisms) refers to single base-pair substitutions in the germline DNA of different individuals that either destroys or creates a new recognition site for a given restriction enzyme.
PCR is used to amplify a specific region of a DNA strand (the DNA target).
FISH is a technique used to identify the presence of specific chromosomes or chromosomal regions through hybridization (attachment) of fluorescently-labeled DNA probes to denatured chromosomal DNA.
Southern blotting combines the transfer of electrophoresis-separated DNA fragments to a filter membrane and subsequent fragment detection by probe hybridization
