Biochemistry Basics

Question Probe used in western blot: A. Antibody B. M-RNA C. DNA D. t-RNA   Show Answer [ads id=”21890″] Correct Answer » A Explanation Western blot (immunoblot) analyses protein by antibody labeled probe. Northern blot analyses m-RNA by a radioactive probe and southern blot analysis DNA by a radioactive probe.

Question The primary structure of a protein refers to: A. Linear structure and order of the amino acids present B. Regular confirmational forms of a protein C. Complete three-dimensional structure of the polypeptide units of a given protein D. Subunit structure of the protein   Show Answer [ads id=”21890″] Correct Answer » A Explanation Ans

Question An amino acid which does not participate in alpha-helix formation is: A. Leucine B. Glycine C. Proline D. Lysine   Show Answer [ads id=”21890″] Correct Answer » C Explanation Ans.C. Proline Different amino-acid sequences have different propensities for forming an α–helical structure. Methionine, alanine, leucine, glutamate, and lysine uncharged (“MALEK” in the amino–acid 1-letter codes) all have especially high helix-forming propensities,

Question Folding of the protein chain is due to : A. Amide bond B. Hydrogen bonds C. Phosphodiesterase bonds D. Disulfide bond   Show Answer [ads id=”21890″] Correct Answer » B Explanation Ans.B. Hydrogen bond Protein folding occurs in a stepwise process. As the polypeptide is being synthesized by the ribosome, the initial segment of

Question An Immunoglobulin molecule represents the following level of organized protein structure: A. Primary structure B. Secondary structure C. Tertiary structure D. Quaternary structure   Show Answer [ads id=”21890″] Correct Answer » D Explanation Ans ‘D’ Quaternary structure Quaternary Structurei. Certain polypeptides will aggregate to form one functional protein.This is referred to as the quaternary

Question True about Isopeptide bond: A. It makes protein resistant. B. Bond is formed between the carboxyl terminus of one protein and the amino group of a lysine residue on another. C. Involves in post-transcriptional modification of protein and enzyme acts as a catalyst for the bond formation both. D. All of the above   Show

Question Stability of the toxoid in snake’s venom is due to: A. Disulfide bond B. Hydrogen bond C. Ionic bond D. Van Der Wall’s bond   Show Answer [ads id=”21890″] Correct Answer » A Explanation Ans ‘A’ Disulfide bond  A disulfide linkage contributes to the stability of the three-dimensional shape of the protein molecule. Many disulfide

Question Trinucleotide repeats are found in: A. Huntington’s disease B. Spinocerebellar ataxia C. Amyotrophic lateral sclerosis D. Parkinson’s disease   Show Answer [ads id=”21890″] Correct Answer » A:B Explanation Trinucleotide expansions In Huntington’s chorea, CAG trinucleotides are repeated 30 to 300 times. This leads to a polyglutamine repeat in the protein. The severity of the

Question There are more than 300 variants of human hemoglobin gene. Among these only a few are fatal. Hence, the most important factor to be conserved in a protein for its function is the: A. Amino acid sequence B. Ligand binding residues C. Structure D. Environment   Show Answer [ads id=”21890″] Correct Answer » B

Question Elongation arrest occurs due to? A. SRP- R B. SRP C. Signal peptide D. Docking protein   Show Answer [ads id=”21890″] Correct Answer » B Explanation Ans ‘B’ SRP Signal recognition particle (SRP) is attached to the SP region, which blocks further protein synthesis. SRP-SP ribosome complex then binds to a docking protein or