A. Multiple origins of replication are possible for bacteria
B. Lagging strand stick by RNA primase
C. DNA replication proceeds in one direction
D. All of the above
Ans.D. All of the above
The helicase or DnaB separates the strands, using energy from ATP. Helicases move on both directions, separating the strands in advance of the replication. This forms a replication bubble with two replication forks.
Single-stranded DNA binding proteins (SSB) stabilize the complex.
The small DNA molecules attached to its own primer RNA are called Okazaki fragments. The synthesis along the lagging strand is in 5′ to 3′ direction. As it moves, the primase synthesizes short RNA primer, to which deoxyribonucleotides are added by DNA polymerase. (DNAP-alpha synthesizes short pieces, which are extended by DNAP-delta. This delta enzyme has a role in the synthesis of both leading and lagging strands).