Question
Identify the biphasic culture medium used for Brucella culture as shown in the photograph below?
A. Bordet Gangou medium.
B. Regan Lowe medium.
C. Castaneda medium.
D. Cary Blair medium.
Show Answer
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Correct Answer » C
Explanation
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Ans:C. Castaneda medium.
The biphasic culture medium used for Brucella culture as shown in the photograph above is Castaneda medium.
- Sometimes, a culture system comprises of both liquid and solid medium in the same bottle. This is known as biphasic medium (Castaneda system for blood culture).
- The inoculum is added to the liquid medium and when subcultures are to be made, the bottle is simply tilted to allow the liquid to flow over the solid medium. This obviates the need for frequent opening of the culture bottle to subculture.
- It is used to grow Brucella from blood.
