ln serum protein electrophoresis, Albumin is represented by?
ln Serum protein electrophoresis as shown in the graph above, Albumin is represented by 5.
Serum protein electrophoresis (SPEP)
- It is a method of separating proteins based on their net charge, size, and shape.
- The 2 major types of protein present in the serum are albumin and the globulin proteins.
- Albumin is the major protein component of serum and represents the largest peak that lies closest to the positive electrode.
- Globulins comprise a much smaller fraction of the total serum protein but represent the primary focus of interpretation of serum protein electrophoresis.
- Five globulin categories are represented: alpha-1, alpha-2, beta-1, beta-2, and gamma, with the gamma fraction being closest to the negative electrode.
Serum Protein Fractions and Conditions Associated with an Increased or Decreased Level
|Serum Protein Fraction||Increased||Decreased|
|Albumin||Severe dehydration||Malnutrition, cachexia, liver disease, nephrotic syndrome, protein-losing enteropathies, severe burns|
|Alpha-1||Inflammatory states, pregnancy||Alpha-1 antitrypsin deficiency|
|Alpha-2||Inflammatory states, nephrotic syndrome, oral contraceptive use, steroid use, hyperthyroidism||Hemolysis, liver disease|
|Beta||Hyperlipidemia, iron-deficiency anemia||Hypo-B-lipoproteinemia, malnutrition|
|Gamma||Polyclonal and Monoclonal Gammopathies||Agammaglobulinemia, hypogammaglobulinemia|