Question
The pathogenecity of Trophozoite shown in the picture below is indicated by ?
A. Isoenzyme pattern.
B. Size.
C. Nuclear pattern.
D. ELISA test.
Show Answer
|
Correct Answer » A Explanation |
 |
The pathogenecity of Entamoeba histolytica is indicated by Isoenzyme pattern.
Zymodene
- There are distinct invasive and noninvasive strains of E. histolytica. These strains vary according to their isoenzyme patterns (zymodemes).
- E. histolytica strains have 22 zymodemes of these 10 zymodemes are invasive and 12 are noninvasive.
- Zymodemes of E. histolytica are identified according to the electrophoretic mobility of 4 enzymes.
- L-malate : NADP+ Oxidoreductase
- Phosphoglucomutase (PGM) → most important
- Glucose-phosphate isomerase
- Hexokinase
- Electrophoresis of PGM can show one or more of the 4 bands : a, 13, y and 5. Strains of E. histolytica showing, an absence of a-band together with the presence of 13 band are virulent i.e., invasive.