Question
Identify the blotting techniques:
A. A:Northern
B:Southern
C:Westen
B. A:Southern
B:Western
C:Northern
C. A:Westen
B:Northern
C:Southern
D. A:Southern
B:Northern
C:Westen
Show Answer
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Correct Answer » D
Explanation
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Ans:D. A:Southern
B:Northern
C:Westen
Types of Blots
| Blot type |
Material Analyzed |
Electrophoresis Required |
Probe Used |
Purpose |
| Southern |
DNA |
Yes |
32 P-DNA |
To determine which restriction fragments of DNA are associated with a particular gene |
| Northern |
RNA |
Yes |
32 P -DNA |
To measure sizes and amounts of specific m-RNA molecules to answer questions about gene expression |
| Western |
Protein |
Yes |
125 I or enzyme linked Antibody |
To measure amount of antigen(proteins) or antibody |
| Dot(Slot) |
RnA,DNA or Protein |
No |
Same as for blots above |
To detect specific DNA,RNA,protein or Antibody. |
