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Image Based Question – 74296

Question

Which of the following Treponema is seen in the silver impregnation micrograph below?

A. Leptospira interrogans

B. Treponema pallidum

C. Ehrlichia chaffeensis

D. Borrelia burgdorferi

 

Show Answer

Correct Answer » A

Explanation

Ans: A.Leptospira interrogans

(Ref Ananthanarayan 10/e p3287, 8/e p372; Jawetz 27/e p330)

  • Hook like appearance of spirochetes in dark field microscopy image – Suggestive of Leptospira infection.

Leptospirae:

  • Tightly coiled, thin, flexible spirochetes 5-15 µm long, with very fine spirals 0.1-0.2 pm wide.
  • One end bent forming hook.
  • Actively motile – Best seen with dark-field microscope.

Electron micrograph image:

  • Thin axial filament & delicate membrane.
  • Spirochete – Delicate.

Dark-field view:

  • Appear only as a chain of minute cocci – Due to extreme delicacy.
  • Does not stain readily.
  • Can be silver-impregnated.
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