Image Based Question – 74873

A patient was suffering from hepatorenal syndrome. A urine sample was obtained from the patient and was examined under the microscope. Which of the following technique is treing used to make the organism visible?

A. Dark field microscopy

B. Routine microscopy with negatively stained background

C. Phase contrast microscopy

D. Compound light microscopy

Correct Answer » A Explanation

Ans. A. Dark field microscopy

• Leptospira are extremely thin, hence seen under dark ground microscope.
• They are tightly and regularly coiled, with characteristic hooked ends like umbrella handle.
• They are highly motile; exhibit spinning and translational movements.