Question
Which of the following methods is NOT used for the measurement of body fluid volumes:
| A. |
Antipyrine for total body water
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| B. |
Inulin for extracellular fluid
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| C. |
Evans blue for plasma volume
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| D. |
125I-albumin for blood volume
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Show Answer
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Correct Answer � D
Explanation
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Ans. D i.e. 125I-albumin for blood volume
- Blood cell volume is determined by the hematocrit value and radioisotope method (mainly Cr52).The isotopes most commonly used are chromium. -51 (labelled red blood cells) and iodine-131 (serum albumin), both γ-emitting isotopes.
MEASUREMENT OF BODY FLUID COMPARTMENTS
- Measurement of body fluid volumes is done by ‘Dilution method’
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VOLUME
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MEASUREMENT
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TBW
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- Heavy water (Deuterium oxide), Tritium oxide, Aminopyrine.
- By dilution principle
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ECF
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- By inulin (most accurate), Sucrose, Mannitol, radioactive sodium, radioactive iodide, Thiosulfate
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PLASMA VOLUME(PV)
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- By using Evans blue (T1824), Serum albumin labeled with radioactive Iodine
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RED CELL VOLUME
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- By RBCs tagged with radioactive Chromium -51, Phosphorus-32, Fe-55 or Fe59
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ICF
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- Can’t be measured directly.
- Formula = (TBW-ECF)
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