A patient was suffering from the hepatorenal syndrome. A urine sample was obtained from the patient and was examined under the microscope. Which of the following techniques is being used to make the organism visible?
Dark field microscopy
Routine microscopy with negatively stained background
Phase contrast microscopy
Compound light microscopy
Ans. A. Dark field microscopy
- Leptospira are extremely thin, hence seen under dark ground microscope.
- They are tightly and regularly coiled, with characteristic hooked ends like umbrella handles.
- They are highly motile; exhibit spinning and translational movements.