Staphylococci

STAPHYLOCOCCI

Q. 1

Match the following:
I. Ogston                    (i) Malta fever
II. Frenkel                   (ii) Spirochaete
III. Bruce                    (iii) Staphylococcus
IV.Schaudinn              (iv) Pneumococcus
 A

I (i) II (iii) III (ii) IV (iv)

 B

I (ii) II (iii) III (i) IV (iv)

 C

I (iii) II (iv) III (i) IV (ii)

 D

I (iii) II (ii) III (i) IV (iv)

Q. 1

Match the following:
I. Ogston                    (i) Malta fever
II. Frenkel                   (ii) Spirochaete
III. Bruce                    (iii) Staphylococcus
IV.Schaudinn              (iv) Pneumococcus
 A

I (i) II (iii) III (ii) IV (iv)

 B

I (ii) II (iii) III (i) IV (iv)

 C

I (iii) II (iv) III (i) IV (ii)

 D

I (iii) II (ii) III (i) IV (iv)

Ans. C

Explanation:

Ogston discovered Staphylococcus in 1881.
Frenkel described Pneumococcus in 1886.
Bruce identified the causative agent of Malta fever in 1887.
Schaudinn and Hoffmann discovered discovered the spirochete of syphilis in 1905.
 
Ref: Textbook of Microbiology By Ananthanarayan And Paniker, 7th edition, Page 2.

Q. 2

How does staphylococcus aureus differ from staphylococcus epidermidis?

 A

It is coagulase positive

 B

It is a common cause of UTI

 C

It forms white colonies on blood agar

 D

It causes endocarditis in drug addicts

Q. 2

How does staphylococcus aureus differ from staphylococcus epidermidis?

 A

It is coagulase positive

 B

It is a common cause of UTI

 C

It forms white colonies on blood agar

 D

It causes endocarditis in drug addicts

Ans. A

Explanation:

Staphylococcus aureus is coagulase positive whereas staphylococcus epidermidis is coagulase negative.
Other coagulase negative staphylococci are S haemolyticus, S saccharolyticus, S hominis, S schleiferi, S lugdunensis and S simulans.

Test

Staphylococcus aureus

Staphylococcus epidermidis

Coagulase

Positive

Negative

Clumping factor

Positive

Negative

Heat stable nuclease

Positive

Negative

Urease

Variable

Negative

Acid from mannitol

Positive

Negative

Acid from trehalose

Positive

Negative

 

Ref: Textbook of Microbiology and Immunology By Parija, Pages 188-190


Q. 3

Staphylococcus aureus and Streptococcus pyogenes are SIMILAR in all of the following features EXCEPT one. Pick the INCORRECT statement:

 A

Both produce numerous exotoxins and exoenzymes

 B

Both are Gram-positive cocci

 C

Both cause skin, tissue, and systemic infections

 D

Both are catalase positive

Q. 3

Staphylococcus aureus and Streptococcus pyogenes are SIMILAR in all of the following features EXCEPT one. Pick the INCORRECT statement:

 A

Both produce numerous exotoxins and exoenzymes

 B

Both are Gram-positive cocci

 C

Both cause skin, tissue, and systemic infections

 D

Both are catalase positive

Ans. D

Explanation:

Both are catalase positive. This is the incorrect pair: S. aureus is catalase-positive, but S. pyogenes (like all streps) is catalase-negative. This is an important point in diagnosis in the lab. 

Also Know:
The group B streptococcus (S agalactiae) is the most common cause of sepsis in newborns and the pneumococcus (S pneumoniae) a leading cause of both pneumonia and meningitis in persons of all ages.
 
Ref: Ray C.G., Ryan K.J. (2010). Chapter 25. Streptococci and Enterococci. In C.G. Ray, K.J. Ryan (Eds), Sherris Medical Microbiology, 5e. 

Q. 4

Staphylococcus is –

 A

Gram – positive cocci

 B

Gram – negative cocci

 C

Gram – positive bacillus

 D

Gram – negative bacillus

Q. 4

Staphylococcus is –

 A

Gram – positive cocci

 B

Gram – negative cocci

 C

Gram – positive bacillus

 D

Gram – negative bacillus

Ans. A

Explanation:

Ans. is ‘a’ i.e., Gram – positive cocci

Gram positive bacteria

.    There are 6 classic grain positive bacteria that cause human disease.

.     Of these 2 are cocci

  1. Staphylococci
  2. Streptococci (including anaerobic peptostreptococci)

.  Other 4 are bacilli

  1. Corynebacterium                         3. Clostridium
  2. Bacillus                                      4. Listeria

Q. 5

All of the following statements are true regarding Staphylococci except –

 A

A majority of infections caused by coagulase­negative Staphylococci are due to Staphylococcus epidermidis

 B

3-lactamase production in Staphylococci is under plasmid control

 C

Expression of methicillin resistance in Staphylococcus aureus increases when it is incubated at 37°C on blood agar.

 D

Methicillin resistance in Staphylococcus aureus is independent of (3-lactamase production

Q. 5

All of the following statements are true regarding Staphylococci except –

 A

A majority of infections caused by coagulase­negative Staphylococci are due to Staphylococcus epidermidis

 B

3-lactamase production in Staphylococci is under plasmid control

 C

Expression of methicillin resistance in Staphylococcus aureus increases when it is incubated at 37°C on blood agar.

 D

Methicillin resistance in Staphylococcus aureus is independent of (3-lactamase production

Ans. C

Explanation:

Ans. is ‘c’ i.e., Expression of methicillin resistance in staph aureus increases when it is incubated at 37°C on blood agar


Q. 6

Staphylococcus differes from streptococcus by ‑

 A

Coagulase test

 B

Catalase test

 C

Phasphatase

 D

Gram negative

Q. 6

Staphylococcus differes from streptococcus by ‑

 A

Coagulase test

 B

Catalase test

 C

Phasphatase

 D

Gram negative

Ans. B

Explanation:

.   Coagulase test is used to differentiate the different species of staphylococci (staph aureus, staph. epidermidis).

.  Catalase test is used to differentiate staphylococci from streptococci.

.     Coagulase test cannot be used to differentiate streptococci from staphylococci because certain species of staphylococci are coagulase negative (coagulase negative staphylococci) as streptococci.

.  But you should keep in mind that staphylococcus aureus (not all specises of staphylococci) can be differentiated from streptococci by coagulae test also as staph. aureus is coagulase positive while streptococci are coagulase negative.


Q. 7

Common natural flora of skin are –

 A

Streptococcus

 B

Staphylococcus aureus

 C

Candida albicans

 D

All

Q. 7

Common natural flora of skin are –

 A

Streptococcus

 B

Staphylococcus aureus

 C

Candida albicans

 D

All

Ans. D

Explanation:

Ans. is ‘a’ i.e., Streptococcus; ‘b’ i.e., Staphylococcus aureus; ‘c’ i.e., Candida albicans

Normal Microbial flora or normal commensal flora or indigenous flora

.Normal microbial flora term is used to describe microorganisms that are frequently found in particlar anatomic sites in healthy individuals.

.   The constitients and numbers of the flora vary in different anatomic sites and sometimes at different ages of life.

.  Perhaps one of the most important roles of the normal microbial flora is to help prevent infectious disease following exposure to potential microbial pathogens. The normal commensal has the physical advantage of previous occupancy, especially on skin and mucous membrane.

.   The normal flora of the intestine plays an important role in human nutrition and metabolism.

Normal microbial flora of skin

Resident

Transient flora

Corynebacteriurn, Micrococcus, Coagulase

Staph aureus, viridans streptococci

negative staphylococci, Propionobacterium,

group A streptococci, Enterococcus

Pityrosporrum, Demodex follicularum

Malassezia, E.coli, Proteus, Klebsiella,

Candida, Trichophyton.



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