A. Kirby Bauer disc diffusion method.
B. Automated Antimicrobial Susceptibility testing system.
C. Broth dilution method.
D. Agar dilution method.
Ans:A. Kirby Bauer disc diffusion method.
Antibiotic Sensitivity test
- Antibiotic sensitivity is the susceptibility of bacteria to antibiotics.
Testing for antibiotic sensitivity is often done by the Kirby-Bauer method.
- Small wafers containing antibiotics are placed onto a plate upon which bacteria are growing. If the bacteria are sensitive to the antibiotic, a clear ring, or zone of inhibition, is seen around the wafer indicating poor growth.
Other methods to test antimicrobial susceptibility include the
- Stokes method(disc diffusion method)
- Etest: (also based on antibiotic diffusion)
- It is a commercially available test that utilizes a plastic test strip impregnated with a gradually decreasing concentration of a particular antibiotic.
Agar and Broth dilution methods for minimum inhibitory concentration (MIC) determination.
- The Broth dilution method involves subjecting the isolate to a series of concentrations of antimicrobial agents in a broth environment.
- A procedure similar to broth dilution is agar dilution. Agar dilution method follows the principle of establishing the lowest concentration of the serially diluted antibiotic concentration at which bacterial growth is still inhibited.
Automated Antimicrobial Susceptibility testing system