This image was taken by attaching the camera to the microscope. What is the requirement for such a microscope?
A. Dark field condenser
B. Phase shifter
C. Dichroic mirror
D. Cathode ray tube
Ans: C. Dichroic mirror
Typical components of fluorescence microscope:
- Light source (xenon/mercury-vapor lamp – common).
- More advanced forms – High-power LEDs & lasers.
- Excitation filter
- Dichroic mirror (or dichroic beam splitter).
- Emission filter.
(Ref Molecular Fluorescence.. Principles and Applications by Bernard Valeta; Mario Nuno Berheran Santos 2nd edition)