Separation techniques of protein
Proteins are separated on the basis of charge in ‑
| A |
SDS-PAGE |
|
| B |
Ultracentrifugation |
|
| C |
Affinity chromatography |
|
| D |
HPLC |
Proteins are separated on the basis of charge in ‑
| A |
SDS-PAGE |
|
| B |
Ultracentrifugation |
|
| C |
Affinity chromatography |
|
| D |
HPLC |
Ans. is ‘d’ i.e., HPLC
Molecular size of protein is determined by‑
| A |
Sedimentation |
|
| B |
SDS-PAGE |
|
| C |
Salting out |
|
| D |
Liophilization |
Molecular size of protein is determined by‑
| A |
Sedimentation |
|
| B |
SDS-PAGE |
|
| C |
Salting out |
|
| D |
Liophilization |
The molecular size of protein is estimated usually by SDS-PAGE.
Other common methods are mass spectroscopy and MALDI.
Which of the following is used to study protein-protein interaction ‑
| A |
Western blot |
|
| B |
Affinity electrophoresis |
|
| C |
Thin-layer chromatography |
|
| D |
None |
Which of the following is used to study protein-protein interaction ‑
| A |
Western blot |
|
| B |
Affinity electrophoresis |
|
| C |
Thin-layer chromatography |
|
| D |
None |
Ans. is ‘b’ i.e., Affinity electrophoresis
- The most conventional and widely used high-through put methods for protein-protein interactions (PPI) are yeast two-hybrid screening and affinity purification coupled to mass spectroscopy.
Other methods used to study PPI are :-
- LI Affinity electrophoresis
- Co-immunoprecipitation
- Protein microarrays
- Analytical ultracentrifugation
- Light scattering
- Fluorescence spectroscopy
- SPINE (strep-protein interaction experiment)
- LUMIER (luminescence-based mammalian intractome mapping)
- Calorimetry
- QUICK (quantitative immunoprecipitation combined with knock-down)
- BiFC (Biomolecular fluorescence complementation)
- PLA (Proximity ligation assay)
- Resonance energy transfer system
- Mammalian protein-protein interaction trap
- Surface plasma resonance
- Protein-fragment complementation assay
- Tandom affinity purification
Which of the following method of protein separation is not dependent on molecular size ‑
| A |
Gel filtration chromatography |
|
| B |
Ultracentrifugation |
|
| C |
Ion-exchange chromatography |
|
| D |
SDS-PAGE |
Which of the following method of protein separation is not dependent on molecular size ‑
| A |
Gel filtration chromatography |
|
| B |
Ultracentrifugation |
|
| C |
Ion-exchange chromatography |
|
| D |
SDS-PAGE |
Ans. is ‘c’ i.e., Ion-exchange chromatography
