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Structure Of Rna

STRUCTURE OF RNA

Q. 1

All of the following statements about Ribozymes are false, EXCEPT:

 A

They are DNA molecules

 B

They are not present in ribosomes

 C

They plays a key role in RNA synthesis

 D

They play a key role in post-transcriptional conversion of pre-mRNA to mature mRNA

Q. 1

All of the following statements about Ribozymes are false, EXCEPT:

 A

They are DNA molecules

 B

They are not present in ribosomes

 C

They plays a key role in RNA synthesis

 D

They play a key role in post-transcriptional conversion of pre-mRNA to mature mRNA

Ans. D

Explanation:

Ribozymes are RNAs which exhibit highly substrate specific catalytic activity.
They play a key role in the intron transcripts excision and exon transcripts splicing events essential for the conversion of pre mRNA to mature mRNA.
 
Ribozymes catalyzed transesterification, and ultimate hydrolysis of phosphodiester bonds in RNA molecules. 
 
Ref: Textbook Of Medical Biochemistry (3Rd Edn.) By S. Ramakrishnan page 338.

Q. 2

mRNA is a complimentary copy of ‑

 A

TRNA

 B

RRNA

 C

Ribosomal DNA

 D

A single strand of DNA

Q. 2

mRNA is a complimentary copy of ‑

 A

TRNA

 B

RRNA

 C

Ribosomal DNA

 D

A single strand of DNA

Ans. D

Explanation:

D i.e. A single strand of DNA


Q. 3

True about ribozymes are A/E

 A

Catalytic activity

 B

Involved in transesterification

 C

Hammerhead metallo enzyme

 D

Deamination

Q. 3

True about ribozymes are A/E

 A

Catalytic activity

 B

Involved in transesterification

 C

Hammerhead metallo enzyme

 D

Deamination

Ans. D

Explanation:

D i.e. Deamination

Ribozyme is RNA with catalytic activityQ. Examples include selfsplicing group I introns, RNase P, & hammerhead metallo enzymeQ of virusoids (requiring Mg2+). These are involved in transesterification, phosphodiester bond hydrolysis (cleavage), RNA metabolism (splicing & endoribonuclease), peptide bond formation (peptidyl transferases) and site specific RNA cleavage.

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Q. 4

Shine-Dalgarno sequence in bacterial mRNA is near:

 A

AUG codon

 B

UAA codon

 C

UAG codon

 D

UGA codon

Q. 4

Shine-Dalgarno sequence in bacterial mRNA is near:

 A

AUG codon

 B

UAA codon

 C

UAG codon

 D

UGA codon

Ans. A

Explanation:

A i.e. AUG Codon


Q. 5

Ribozymes is/are :

 A

Splicing of heterogenous RNA (hnRNA) to form mRNA

 B

Splicing of polypeptide chain and mRNA

 C

Transcription of mRNA

 D

All

Q. 5

Ribozymes is/are :

 A

Splicing of heterogenous RNA (hnRNA) to form mRNA

 B

Splicing of polypeptide chain and mRNA

 C

Transcription of mRNA

 D

All

Ans. A

Explanation:

Q. 6

Poly ‘A’ tail attached at 3′ end of mRNA helps in‑

 A

Unwinding of mRNA

 B

Stabilization of mRNA

 C

Polymerization of mRNA

 D

Transcription of mRNA

Q. 6

Poly ‘A’ tail attached at 3′ end of mRNA helps in‑

 A

Unwinding of mRNA

 B

Stabilization of mRNA

 C

Polymerization of mRNA

 D

Transcription of mRNA

Ans. B

Explanation:

Ans. is ‘b’ i.e., Stabilization of mRNA

At the 51-end, mRNA possesses a 7-methylguanosine triphosphate cap which helps in the recognition of mRNA in protein biosynthesis and it helps to stabilize the mRNA by preventing attack of 51-exonuclease.

At its 31-end, there is a poly-A tail made up of several adenylate residues which stabilize mRNA by preventing attack of 31-exonuclease.

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Q. 7

3′ end of t-RNA posseses ‑

 A

Poly ‘A’ tail

 B

CCA sequence

 C

Anticodon

 D

D arm

Q. 7

3′ end of t-RNA posseses ‑

 A

Poly ‘A’ tail

 B

CCA sequence

 C

Anticodon

 D

D arm

Ans. B

Explanation:

 

t-RNA molecule get folded into a structure that appears like a clover leaf.

There are four arms.

  1. Acceptor arm : It consists of a base paired stem that terminates in the sequence CCA at the 3′ end. This is the attachment site for amino acids.
  2. D arm – It contains the base dihydrouridine (D).
  3.  Anticodon arm – It contains anticodon that base pairs with the codon of coming mRNA. Anticodon has nucleotide sequence complementary to the codon of mRNA and is responsible for the specificity of the t RNA.
  4. Tyr C arm : It contains both ribothymidine (T) and pseudouridine

Q. 8

True about tRNA ‑

 A

80% of total RNA

 B

Contains 50-60 nucleotides

 C

CCA sequence is transcribed

 D

Longest RNA

Q. 8

True about tRNA ‑

 A

80% of total RNA

 B

Contains 50-60 nucleotides

 C

CCA sequence is transcribed

 D

Longest RNA

Ans. C

Explanation:

 

“The CCA tail is a CCA sequence at 3′ end of the tRNA molecule. In prokaryotes, CCA sequence is transcribed. In eukaryotes, the CCA sequence is added during processing”.

“tRNA is the smallest of three major species of RNAs”   — Dinesh puri

tRNA comprises 15% of total RNA in the cell. It contains 73-93 nucleotide residue.


Q. 9

Both DNA and RNA are present in:

 A

Bacteria

 B

Prions

 C

Virioids

 D

Plasmid

Q. 9

Both DNA and RNA are present in:

 A

Bacteria

 B

Prions

 C

Virioids

 D

Plasmid

Ans. A

Explanation:

Ans. a. Bacteria

Both DNA and RNA are pre sent in Bacteria

Micro-organism Genetic Material
Bacteria Contain both DNA and RNA, as well as extra-chromosomal DNA material (plasmids)Q
Plasmid

Extra-choromosomal circular DNA present in cytoplasm of bacteria and capable of autonomous

Viroids Contain low molecular weight RNA (No DNA)Q
Prions

Are misfolded proteinsQ

Devoid of both DNA and RNA

 

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Q. 10

Synthesis of rRNA takes place in

 A

Cytosol

 B

Nucleus

 C

Nucleolus

 D

Mitochondria

Q. 10

Synthesis of rRNA takes place in

 A

Cytosol

 B

Nucleus

 C

Nucleolus

 D

Mitochondria

Ans. C

Explanation:

Ans. is ‘c i.e., Nucleolus 

Organelle

Function

Nucleolus Site of synthesis of r-RNA
Ribosomes

Site of protein synthesis, translation of mRNA

RER / Site of protein synthesis
Granular ER

(e.g. hormones, proteins found in enzyme)

SER / Agrannlar

Site of steroid synthesis I0

ER detoxification / FA elongation

Golgi Body

Processing / packaging, intracellular sorting of proteins, formation of lysosomes
Lysosomes Contain digestive / lytic enzymes and hydrolases (suicidal bags of cell)
Peroxisomes

Contain oxidases


Q. 11

RNA which contains codon for speicific amino acid ‑

 A

tRNA

 B

rRNA

 C

mRNA

 D

None

Q. 11

RNA which contains codon for speicific amino acid ‑

 A

tRNA

 B

rRNA

 C

mRNA

 D

None

Ans. C

Explanation:

Ans. is ‘c’ i.e., m RNA

The m RNA carries genetic information in the form of codons.

  • Codons are a group of three adjacent nucleotides that code for the amino acids of protein.
  • Each mRNA molecule is a transcript of antisense or template strand of a particular gene.
  • Its nucleotide sequence is complementary to that of antisense or template strand of the gene, i.e. adenine for thyamine, guanine for cytosine, uracil for adenine (as RNA does not contain thymine) and cytosine for guanine.
  • For example, if antisense strand of DNA has a gene with sequence 5′-TTACGTAC-3′, its complementary RNA transcript will be 5 ‘-GUACGUAA-3’.

Q. 12

RNA is present in ‑

 A

Cytoplasm

 B

Nucleus

 C

Ribosome

 D

All of the above

Q. 12

RNA is present in ‑

 A

Cytoplasm

 B

Nucleus

 C

Ribosome

 D

All of the above

Ans. D

Explanation:

Ans. is `d’ i.e., All of the above

  • mRNA is synthesized from DNA by the process of transcription in the nucleus. 
  • After formation mRNA transport out of the nucleus into cytoplasm.
  • t-RNA is also synthesized in nucleus and is transported to cytoplasm.
  • Protein synthesis (translation) occurs in ribosomes, and requires both mRNA and tRNA.
  • rRNA is present in ribosomes.
  • rRNA is synthesized in nucleolus

Thus, RNA can be found in –

  1. Nucleus
  2. Cytoplasm
  3. Ribosome
  4. Nucleolus

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