STRUCTURE OF RNA
All of the following statements about Ribozymes are false, EXCEPT:
A |
They are DNA molecules |
|
B |
They are not present in ribosomes |
|
C |
They plays a key role in RNA synthesis |
|
D |
They play a key role in post-transcriptional conversion of pre-mRNA to mature mRNA |
All of the following statements about Ribozymes are false, EXCEPT:
A |
They are DNA molecules |
|
B |
They are not present in ribosomes |
|
C |
They plays a key role in RNA synthesis |
|
D |
They play a key role in post-transcriptional conversion of pre-mRNA to mature mRNA |
mRNA is a complimentary copy of ‑
A |
TRNA |
|
B |
RRNA |
|
C |
Ribosomal DNA |
|
D |
A single strand of DNA |
mRNA is a complimentary copy of ‑
A |
TRNA |
|
B |
RRNA |
|
C |
Ribosomal DNA |
|
D |
A single strand of DNA |
D i.e. A single strand of DNA
True about ribozymes are A/E
A |
Catalytic activity |
|
B |
Involved in transesterification |
|
C |
Hammerhead metallo enzyme |
|
D |
Deamination |
True about ribozymes are A/E
A |
Catalytic activity |
|
B |
Involved in transesterification |
|
C |
Hammerhead metallo enzyme |
|
D |
Deamination |
D i.e. Deamination
Ribozyme is RNA with catalytic activityQ. Examples include selfsplicing group I introns, RNase P, & hammerhead metallo enzymeQ of virusoids (requiring Mg2+). These are involved in transesterification, phosphodiester bond hydrolysis (cleavage), RNA metabolism (splicing & endoribonuclease), peptide bond formation (peptidyl transferases) and site specific RNA cleavage.
Shine-Dalgarno sequence in bacterial mRNA is near:
A |
AUG codon |
|
B |
UAA codon |
|
C |
UAG codon |
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D |
UGA codon |
Shine-Dalgarno sequence in bacterial mRNA is near:
A |
AUG codon |
|
B |
UAA codon |
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C |
UAG codon |
|
D |
UGA codon |
A i.e. AUG Codon
Ribozymes is/are :
A |
Splicing of heterogenous RNA (hnRNA) to form mRNA |
|
B |
Splicing of polypeptide chain and mRNA |
|
C |
Transcription of mRNA |
|
D |
All |
Ribozymes is/are :
A |
Splicing of heterogenous RNA (hnRNA) to form mRNA |
|
B |
Splicing of polypeptide chain and mRNA |
|
C |
Transcription of mRNA |
|
D |
All |
Poly ‘A’ tail attached at 3′ end of mRNA helps in‑
A |
Unwinding of mRNA |
|
B |
Stabilization of mRNA |
|
C |
Polymerization of mRNA |
|
D |
Transcription of mRNA |
Poly ‘A’ tail attached at 3′ end of mRNA helps in‑
A |
Unwinding of mRNA |
|
B |
Stabilization of mRNA |
|
C |
Polymerization of mRNA |
|
D |
Transcription of mRNA |
Ans. is ‘b’ i.e., Stabilization of mRNA
At the 51-end, mRNA possesses a 7-methylguanosine triphosphate cap which helps in the recognition of mRNA in protein biosynthesis and it helps to stabilize the mRNA by preventing attack of 51-exonuclease.
At its 31-end, there is a poly-A tail made up of several adenylate residues which stabilize mRNA by preventing attack of 31-exonuclease.
3′ end of t-RNA posseses ‑
A |
Poly ‘A’ tail |
|
B |
CCA sequence |
|
C |
Anticodon |
|
D |
D arm |
3′ end of t-RNA posseses ‑
A |
Poly ‘A’ tail |
|
B |
CCA sequence |
|
C |
Anticodon |
|
D |
D arm |
t-RNA molecule get folded into a structure that appears like a clover leaf.
There are four arms.
- Acceptor arm : It consists of a base paired stem that terminates in the sequence CCA at the 3′ end. This is the attachment site for amino acids.
- D arm – It contains the base dihydrouridine (D).
- Anticodon arm – It contains anticodon that base pairs with the codon of coming mRNA. Anticodon has nucleotide sequence complementary to the codon of mRNA and is responsible for the specificity of the t RNA.
- Tyr C arm : It contains both ribothymidine (T) and pseudouridine
True about tRNA ‑
A |
80% of total RNA |
|
B |
Contains 50-60 nucleotides |
|
C |
CCA sequence is transcribed |
|
D |
Longest RNA |
True about tRNA ‑
A |
80% of total RNA |
|
B |
Contains 50-60 nucleotides |
|
C |
CCA sequence is transcribed |
|
D |
Longest RNA |
“The CCA tail is a CCA sequence at 3′ end of the tRNA molecule. In prokaryotes, CCA sequence is transcribed. In eukaryotes, the CCA sequence is added during processing”.
“tRNA is the smallest of three major species of RNAs” — Dinesh puri
tRNA comprises 15% of total RNA in the cell. It contains 73-93 nucleotide residue.
Both DNA and RNA are present in:
A |
Bacteria |
|
B |
Prions |
|
C |
Virioids |
|
D |
Plasmid |
Both DNA and RNA are present in:
A |
Bacteria |
|
B |
Prions |
|
C |
Virioids |
|
D |
Plasmid |
Ans. a. Bacteria
Both DNA and RNA are pre sent in Bacteria
Micro-organism | Genetic Material |
Bacteria | Contain both DNA and RNA, as well as extra-chromosomal DNA material (plasmids)Q |
Plasmid |
Extra-choromosomal circular DNA present in cytoplasm of bacteria and capable of autonomous |
Viroids | Contain low molecular weight RNA (No DNA)Q |
Prions |
Are misfolded proteinsQ Devoid of both DNA and RNA |
Synthesis of rRNA takes place in ‑
A |
Cytosol |
|
B |
Nucleus |
|
C |
Nucleolus |
|
D |
Mitochondria |
Synthesis of rRNA takes place in ‑
A |
Cytosol |
|
B |
Nucleus |
|
C |
Nucleolus |
|
D |
Mitochondria |
Ans. is ‘c‘ i.e., Nucleolus
Organelle |
Function |
Nucleolus | Site of synthesis of r-RNA |
Ribosomes |
Site of protein synthesis, translation of mRNA |
RER / | Site of protein synthesis |
Granular ER |
(e.g. hormones, proteins found in enzyme) |
SER / Agrannlar |
Site of steroid synthesis I0 |
ER | detoxification / FA elongation |
Golgi Body |
Processing / packaging, intracellular sorting of proteins, formation of lysosomes |
Lysosomes | Contain digestive / lytic enzymes and hydrolases (suicidal bags of cell) |
Peroxisomes |
Contain oxidases |
RNA which contains codon for speicific amino acid ‑
A |
tRNA |
|
B |
rRNA |
|
C |
mRNA |
|
D |
None |
RNA which contains codon for speicific amino acid ‑
A |
tRNA |
|
B |
rRNA |
|
C |
mRNA |
|
D |
None |
Ans. is ‘c’ i.e., m RNA
The m RNA carries genetic information in the form of codons.
- Codons are a group of three adjacent nucleotides that code for the amino acids of protein.
- Each mRNA molecule is a transcript of antisense or template strand of a particular gene.
- Its nucleotide sequence is complementary to that of antisense or template strand of the gene, i.e. adenine for thyamine, guanine for cytosine, uracil for adenine (as RNA does not contain thymine) and cytosine for guanine.
- For example, if antisense strand of DNA has a gene with sequence 5′-TTACGTAC-3′, its complementary RNA transcript will be 5 ‘-GUACGUAA-3’.
RNA is present in ‑
A |
Cytoplasm |
|
B |
Nucleus |
|
C |
Ribosome |
|
D |
All of the above |
RNA is present in ‑
A |
Cytoplasm |
|
B |
Nucleus |
|
C |
Ribosome |
|
D |
All of the above |
Ans. is `d’ i.e., All of the above
- mRNA is synthesized from DNA by the process of transcription in the nucleus.
- After formation mRNA transport out of the nucleus into cytoplasm.
- t-RNA is also synthesized in nucleus and is transported to cytoplasm.
- Protein synthesis (translation) occurs in ribosomes, and requires both mRNA and tRNA.
- rRNA is present in ribosomes.
- rRNA is synthesized in nucleolus
Thus, RNA can be found in –
- Nucleus
- Cytoplasm
- Ribosome
- Nucleolus