CENTRAL DOGMA OF MOLECULAR BIOLOGY Q. 1 Assertion : Central dogma is the flow of information from DNA to mRNA and then decoding the information present in mRNA in the form of protein. Reason : In retroviruses, reverse of central dogma occurs. A If both the assertion and the reason are true and the reason is […]
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MOLECULAR CYTOGENETIC TECHNIQUE- (FISH) FISH is a cytogenetic technique that can be used to detect the presence or absence ofspecific DNA sequences (specific gene locus). fluorescent probes bind to only those parts of the chromosome with which they show a high degree of sequence similarity.like lncRNA, mRNA, or miRNA of interest FISH can be used for rapid identification of chromosome
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MOLECULAR CYTOGENETIC TECHNIQUE- (FISH) Q. 1 Which of these is not a method for introducing genes into a cell? A Transfection B Electroporation C FISH D Recombination Q. 1 Which of these is not a method for introducing genes into a cell? A Transfection B Electroporation C FISH D Recombination Ans. C Explanation: Methods for
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RECOMBINANT DNA TECHNOLOGY RECOMBINANT DNA TECHNOLOGY when a gene of one species is transferred to another living organism, it is called recombinant DNA technology(genetic engineering). It involves isolation and manipulation of DNA to make chimeric or hybrid DNA. Chimeric (hybrid) DNA is a recombinant DNA containing genes from two different species, e g. molecule containing both human and bacterial
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RECOMBINANT DNA TECHNOLOGY Q. 1 Which of the following statements describing restriction endonucleases is TRUE? A They always yield blunt ends B They recognize methylated DNA sequence C They recognize triplet repeats D They cleave both strands in duplex DNA Q. 1 Which of the following statements describing restriction endonucleases is TRUE? A They always
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REAL TIME PCR Real Time PCR- Principle, Process, Markers, Advantages, Applications Real Time PCR is a technique used to monitor the progress of a PCR reaction in real time. At the same time, a relatively amount of PCR product (DNA, cDNA or RNA) can be quantified. Real Time PCR is based on the detection of
Real Time PCR Q. 1 Real Time PCR is used for: A Multiplication of RNA B Multiplication of specific segments of DNA C Multiplication of Proteins D To know how much amplification of DNA has occurred Q. 1 Real Time PCR is used for: A Multiplication of RNA B Multiplication of specific segments of DNA
Difference between Meningitis and Encephalitis S.N. Character Meningitis Encephalitis 1. Definition Inflammation of the thin membranes that surround the brain and spinal cord called the meninges (mostly of the pia mater and arachnoid mater). Inflammation of the brain parenchyma. 2. Causative Agent Bacteria, Viruses and Fungi Mostly Viruses 3. Microorganisms involved The most common are echovirus,
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Difference between Meningitis and Encephalitis Q. 1 Drug used to treat Cryptococcal meningitis is: March 2007 A Pentostatin B Amphotericin B C Zidovudine D Clotrimazole Q. 1 Drug used to treat Cryptococcal meningitis is: March 2007 A Pentostatin B Amphotericin B C Zidovudine D Clotrimazole Ans. B Explanation: Ans. B: Amphotericin B Cryptococcus neoformans is
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Types of culture media-classification Types of culture media The food material or substances required for growing microorganisms in vitro (outside the body) is called culture medium. It is important to grow microorganisms outside the body for the following purposes: 1. to identify the cause of infection from the clinical sample, so that proper treatment can
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